Regulatory
Part:BBa_K4202028:Design
Designed by: Zhijian Yan Group: iGEM22_ZJU-China (2022-10-03)
Pveg-5X leader RNA for Bacillus subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Recombination of Pveg with the leader RNA of PsacB was designed to enhance promoter activity while reducing leaky expression.
The spacer sequence is desigened using online tools(http://crispr.dfci.harvard.edu/SSC/).
Source
The original sequence of leader RNA is obtained from subtiwiki (http://subtiwiki.uni-goettingen.de/v4/gene?id=AAA944BE7F01CE90F7730EECA16F3E4ED77D165A). The sequence of leader RNA is obtained from genomic DNA of Bacillus subtilis through PCR.The original sequence of Pveg is obtained from BBa_K1364008(https://parts.igem.org/Part:BBa_K1364008).